Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: Replacing saturated fat with unsaturated fat in western diet reduces foamy monocytes and atherosclerosis in male Ldlr –/– mice

doi: 10.1161/ATVBAHA.119.313078

Figure Lengend Snippet: EVOND compared to WD reduced foamy monocyte formation and inflammation in Ldlr–/– mice. A, Monocyte frequency in total leukocytes of mice on ND, WD, and EVOND (n=12–20/group). B, Side scatter (SSC) value and Nile red mean fluorescence intensity (MFI) of circulating monocytes of mice on different diets (3 months on diets for Nile red staining). C, Representative fluorescence-activated cell sorter (FACS) examples showing foamy monocytes in blood of Ldlr–/– mice on different diets (left panel). Monocytes (CD115+) were divided into two subsets based on CD36. Elevations in SSC indicated lipid accumulation and foamy monocyte formation; quantification of SSC values of CD36– and CD36+ monocytes in Ldlr–/– mice on diets (n=9–18/group; right panel). D, CD11c expression on CD36– and CD36+ monocytes in Ldlr–/– mice on diets (n=9–18/group). E, Expression of TNFα and IL-1β in monocytes of Ldlr–/– mice on diets (n=4/group). Data are shown as mean±SEM. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: 21 Antibodies and fluorescence-activated cell sorter (FACS) analysis of circulating monocytes For FACS analysis, monoclonal antibodies against the following mouse antigens were used: CD115 (PE, AFS98, eBioscience), CD204 (FITC, 2F8, Bio-Rad Laboratories), CD11c (PerCP-Cy5.5, N418, eBioscience), CD36 (FITC, MF3, Bio-Rad Laboratories), Ly-6C (APC, HK1.4, eBioscience), TNFα (PE, MP6-XT22, eBioscience), and IL-1β (PE, NJTEN3, eBioscience).

Techniques: Fluorescence, Staining, Expressing

Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: Replacing saturated fat with unsaturated fat in western diet reduces foamy monocytes and atherosclerosis in male Ldlr –/– mice

doi: 10.1161/ATVBAHA.119.313078

Figure Lengend Snippet: EVOND reduced monocyte CD36 expression and oxidized LDL (oxLDL) uptake. A, SSC value of monocytes (from ND-fed Ldlr–/– mice) after incubation with triglyceride-rich lipoprotein (TGRL) fraction from mice on WD or EVOND (n=6/group). B, Expression level of CD36 on circulating monocytes from mice on different diets (left panel; n=9–15 mice/group) or on monocytes from ND-fed Ldlr–/– mice after incubation with TGRL fraction from mice on WD or EVOND (right panel; n=6/group). C, Monocyte uptake of DiI-oxLDL. Total leukocytes from Ldlr–/– mice on WD or EVOND were incubated ex vivo with DiI-oxLDL in the absence or presence of anti-mouse CD36 antibody for 1 h, and DiI signals representing monocyte uptake of oxLDL were examined by FACS (n=4/group). Data are shown as mean±SEM. *p<0.05, ***p<0.001.

Article Snippet: 21 Antibodies and fluorescence-activated cell sorter (FACS) analysis of circulating monocytes For FACS analysis, monoclonal antibodies against the following mouse antigens were used: CD115 (PE, AFS98, eBioscience), CD204 (FITC, 2F8, Bio-Rad Laboratories), CD11c (PerCP-Cy5.5, N418, eBioscience), CD36 (FITC, MF3, Bio-Rad Laboratories), Ly-6C (APC, HK1.4, eBioscience), TNFα (PE, MP6-XT22, eBioscience), and IL-1β (PE, NJTEN3, eBioscience).

Techniques: Expressing, Incubation, Ex Vivo

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: The Role of CD36 in the Effect of Arginine in Atherosclerotic Rats

doi: 10.12659/MSM.893388

Figure Lengend Snippet: Representative photomicrograph of CD36 expression with immunohistochemistry in each group rats (200×). ( A ) Control group; ( B ) fat diet group; ( C ) simvastatin group; ( D ) arginine group.

Article Snippet: After blocking with 2% BSA in Tris-buffered saline at room temperature for 1 h, the membranes were incubated with an antibody against rabbit anti-rat CD36 antibody (Wuhan EIAab Science Co., Ltd., Wuhan, China) overnight at 4°C.

Techniques: Expressing, Immunohistochemistry

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: The Role of CD36 in the Effect of Arginine in Atherosclerotic Rats

doi: 10.12659/MSM.893388

Figure Lengend Snippet: Real-time RT-PCR amplification curves of β-actin RNA and CD36 mRNA. This shows that there is no pollution and interference of physical factors in the specimen. The standard is distributed uniformly and its gradient is normal.

Article Snippet: After blocking with 2% BSA in Tris-buffered saline at room temperature for 1 h, the membranes were incubated with an antibody against rabbit anti-rat CD36 antibody (Wuhan EIAab Science Co., Ltd., Wuhan, China) overnight at 4°C.

Techniques: Quantitative RT-PCR, Amplification

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: The Role of CD36 in the Effect of Arginine in Atherosclerotic Rats

doi: 10.12659/MSM.893388

Figure Lengend Snippet: (A) CD36 protein expression of mononuclear cells with Western blot analysis in each group. ( B ) The ratio of CD36 mRNA expression and β-actin RNA expression with RT-PCR in each group.

Article Snippet: After blocking with 2% BSA in Tris-buffered saline at room temperature for 1 h, the membranes were incubated with an antibody against rabbit anti-rat CD36 antibody (Wuhan EIAab Science Co., Ltd., Wuhan, China) overnight at 4°C.

Techniques: Expressing, Western Blot, RNA Expression, Reverse Transcription Polymerase Chain Reaction